| 在有机溶剂中猪胰脂肪酶催化合成己酸乙酯的研究 |
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| 引用本文： | 张艳,何丽明,蒋群,赵凤生. 在有机溶剂中猪胰脂肪酶催化合成己酸乙酯的研究[J]. 精细化工, 2010, 27(2) |
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| 作者姓名： | 张艳 何丽明 蒋群 赵凤生 |
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| 作者单位： | 1. 上海交通大学,药学院,上海,200240
2. 上海交通大学,生命科学技术学院,上海,200240 |
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| 摘 要： | 研究了有机溶剂中猪胰脂肪酶催化正己酸和无水乙醇合成己酸乙酯的反应。以正己酸和无水乙醇在各种反应介质中进行酶催化反应,用气相色谱检测生成的己酸乙酯,考察反应介质、底物浓度、底物摩尔比、加酶量、反应时间对产率的影响。发现在高底物浓度和低底物浓度的条件下,有机溶剂介质对反应的影响是不同的。采用中等底物浓度,选择lgP值适中的溶剂为介质最合适。适当的反应条件为:以环己烷为反应介质,正己酸浓度0.6mol/L,n(正己酸)∶n(无水乙醇)=1∶1.25,加酶量15g/L,反应温度37℃,反应24h后,产率达到90.5%。该文的底物浓度较高,是文献[1,10,11,17]报道的1.5～3.0倍,获得了相近的产率。
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| 关 键 词： | 有机溶剂 生物催化 脂肪酶 己酸乙酯 气相色谱 |
Synthesis of Ethyl Caproate Catalyzed by Pocine Pancreas Lipase in Organic Solvents |
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| ZHANG Yan,HE Li-ming,JIANG Qun,ZHAO Feng-sheng. Synthesis of Ethyl Caproate Catalyzed by Pocine Pancreas Lipase in Organic Solvents[J]. Fine Chemicals, 2010, 27(2) |
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| Authors: | ZHANG Yan HE Li-ming JIANG Qun ZHAO Feng-sheng |
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| Affiliation: | 1.School of Pharmacy/a>;Shanghai Jiao Tong University/a>;Shanghai 200240/a>;China/a>;2.College of Life Science and Biotechnology/a>;China |
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| Abstract: | The synthesis of ethyl caproate from caproic acid and anhydrous ethanol catalyzed by pocine pancreas lipase in organic solvent was investigated.The biotransformation was carried out in several kinds of reaction media.The produced ethyl caproate was determined by gas chromatography.The effects of reaction media,substrate concentration,mole ratio of substrates,enzyme amount and reaction time on the conversion yield were investigated.It was found that the influences of organic solvent on the enzymatic reaction... |
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| Keywords: | organic solvent biocatalysis lipase ethyl caproate gas chromatography |
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