氮掺杂碳纳米粒子荧光共振能量转移测定核黄素

以柠檬酸和乙二胺为前驱体,在油酸介质中通过微波辅助法一步合成了氮掺杂荧光碳纳米粒子,量子产率达41.5%。碳纳米粒子与核黄素可发生有效的荧光共振能量转移,基于此建立了一种定量测定核黄素含量的新方法。测定核黄素含量的线性范围为0~20μg/m L,检出限为68.5 ng/m L。该方法简单、快速、灵敏度高,可用于实际样品中核黄素含量的测定。

Fabrication of Nitrogen-doped Carbon Nanoparticles and Application in the Detection of Riboflavin Based on Fluorescence Resonance Energy Transfer

Nitrogen-doped carbon nanoparticles (N-CNPs) with a fluorescence quantum yield of 41.5％ were prepared from ethylenediamine and citric acid in oleic acid medium by a facile microwave-assisted solvothermal method.A new approach for quick and sensitive determination of riboflavinin was developed based on fluorescence resonance energy transfer from N-CNPs to riboflavinin.There exists a good linear correlation between fluorescence signal and the concentration of riboflavin in the range of 0 ～ 20 μg/mL.The limit of detection (LOD) was estimated to be 68.5 ng/mL based on three times the standard deviation rule.This method can be applied to the determination of riboflavin in real samples.