阿魏酸哌嗪在铁氰化钾体系中的显色反应研究

研究了阿魏酸哌嗪在铁氰化钾体系中氧化还原反应及显色反应,建立了一种快速、简便测定阿魏酸哌嗪的分光光度法。实验利用阿魏酸哌嗪可将Fe(Ⅲ)还原、生成的Fe(Ⅱ)与K_3Fe(CN)_6]反应生成蓝色化合物的原理,通过在其最大吸收波长755 nm处测定体系的吸光度,从而计算阿魏酸哌嗪的含量,同时探讨其反应机理及反应的动力学性质。阿魏酸哌嗪浓度在0.080~17.183 mg/L范围内与吸光度呈良好的线性关系,得其线性回归方程为A=0.029 37+0.14147c(mg/L,r=0.999 1),表观摩尔吸光系数ε为6.71×10~4L/(mol·cm),方法的检出限和精密度分别为0.039 5 mg/L和0.76%。将该方法应用于阿魏酸哌嗪片中阿魏酸哌嗪含量的测定,加标后平均回收率为99.7%。

Chromogenic Reaction of Piperazine Ferulate in the System of Potassium Ferricyanide

The redox reaction and chromogenic reaction of piperazine ferulate in the system of potassium ferricyanide have been investigated by spectrophotometric method.A simple and rapid method for the determination of piperazine ferulate was established.It was based on the fact that Fe(Ⅱ) generated from Fe(Ⅲ) using the reducibility of piperazine ferulate could react with potassium ferricyanide to form the blue compound.Then,the absorbance of the compound was determined at the maximal absorption wavelength of 755 nm and the content of piperazine ferulate was calculated.The reaction mechanism and kinetic property were elucidated intensively.Beer's law was obeyed in the range of 0.080 ～ 17.183 mg/L of piperazine ferulate.The equation of linear regression equation was A =0.029 37 +0.141 47c(mg/L) with a correlation coefficient of 0.999 1 and the apparent molar absorption coefficient of 6.71 × 104 L/(mol* cm).The detection limit was 0.039 5 mg/L,while RSD was 0.76％.This proposed method has been applied to the determination of piperazine ferulate in tablets,with average recovery rate of 99.7％.