一种新型罗丹明类双通道荧光探针及其对Fe~(3+)和Cu~(2+)的选择性识别研究

以罗丹明B、水合肼和吡啶-2-甲醛为反应物,合成了一种新型的罗丹明类Fe~(3+)和Cu~(2+)双通道分子探针(Fluorescent probe,FP),并用核磁和质谱对其分子结构进行了表征。利用荧光分光光度计和紫外可见分光光度计研究了FP对Fe~(3+)、Cu~(2+)等14种金属离子的识别性能。研究结果表明,在V(CH_3OH)∶V(H_2O)=1∶1体系中,当选用荧光光谱分析时,探针FP对Fe~(3+)具有良好的选择性响应,且Fe~(3+)浓度在2~30μmol/L时,探针荧光强度与Fe~(3+)浓度呈线性关系,线性相关系数为0.998。当选用紫外可见光谱分析时,探针FP对Cu~(2+)具有很好的选择性响应,且当Cu~(2+)浓度为0~9μmol/L时,探针FP的吸光度与Cu~(2+)浓度呈线性关系,线性相关系数为0.999。

Synthesis of New Dual Fluorescent Probe Based on Rhodamineand Performance in Selective Recognition of Fe3+ and Cu2+

A novel Rhodamine-based dual fluorescent probe (FP) for detecting Fe3+ and Cu2+ was designed and synthesized using Rhodamine B,hydrazine hydrate and pyridine-formaldehyde as raw reactants.The molecule structure of FP was confirmed by 1HNMR and MS,and the selective recognition properties in detecting Fe3+,Cu2+ and other 12 different kinds of metal ions were investigated by fluorescent spectroscopy and UV-Vis spectroscopy.The results revealed that FP had a high selectivity and sensitivity for Fe3+ using fluorescence spectra,and for Cu2+ via UV-Vis absorption spectra in V(CH3OH):V(H20) =1:l.When the concentration of Fe3+ and Cu2+ was respectively in 2～ 30 μmol/L and 0 ～ 9 μmol/L,there was a good linearity relationship between the targeted metal ion of Fe3+ and Cu2+ and their fluorescence intensity and UV-Vis absorption,and the coefficient values were more than O.998.