二噁英半抗原中间体的合成研究

二噁英类化合物是一类重要的持久性有机污染物,它对环境及人体的危害引起了广泛关注。酶联免疫分析(ELISA)技术是实现高效检测环境中二噁英残留的重要方法,而酶联免疫分析的关键基础是半抗原的合成。对二噁英半抗原合成中的核心步骤进行了详尽地探索,确定了此类反应的最佳反应条件:常温、甲苯为溶剂、0.015 mol氢化钠为碱、催化剂15-冠-5-醚的加入量为n(15-冠-5-醚)∶n(4-氯-3-硝基苯甲醛)=0.02∶1。

Synthesis of Dioxins Haptens Intermediate Product

Dioxins is a kind of important persistent organic pollutants,and it arouses people's concern increasingly because of its harm to environment and human body.Enzyme-linked immunoassay (ELISA) technology is an important method to detect residual dioxins in the environment,and its key step is the synthesis of haptens.In this work,the reaction conditions of this key step have been explored and determined.The optimum reaction conditions were at room temperature,toluene as the solvent,amount of sodium hydride is 0.015 mol,n (15-crown-5 ether) ∶n(4-chloro-3-nitrobenzaldehyde)=0.02∶1.