高效液相色谱法测定白蚁防治药土屏障中的吡虫啉和伊维菌素

建立了测定白蚁防治药土屏障中的吡虫啉和伊维菌素的高效液相色谱法。目标物通过甲醇提取后,经SPE小柱净化,超高效液相色谱分离测定,分别进行了两个添加水平的加标回收实验。吡虫啉平均回收率:粘土96.05%~99.28%,RSD为2.60%~3.26%;砂土82.16%~83.55%,RSD为2.00%~2.28%。伊维菌素平均回收率:粘土92.99%~110.56%,RSD为2.31%~4.69%;砂土101.63%~101.70%,RSD为2.59%~5.12%。该方法具有简单、灵敏度高、重现性好等优点。

Determination of Imidacloprid and Ivermectin in Chemical Soil Barrier by HPLC

To develop methods for determination of imidacloprid and ivermectin in chemical soil barrier by HPLC.By extraction with methanol,luther cleanup was performed on ENVI-18 SPE column.The connent of imidacloprid and ivermectin were determined by HPLC.Tests for recovery were made by addition of two target compounds at two different concentration levels to two kinds of soils.The mean recoveries of imidacloprid and ivermectin in clay soil ranged from 96.05％ to 99.28％ and 92.99％ to 110.56％,and RSD were 2.60％ to 3.26％ (n =6),and 2.31％ to 4.69％ (n =6),respectively.The mean recoveries of imidacloprid and ivermectin in sand soil ranged from 82.16％ to 83.55％ and 101.63％ to 101.70％,and RSD were 2.00％ to 2.28％(n =6),and 2.59％ to 5.12％ (n =6),respectively.The methods are simple,high sensitivity and good reproducibility.