光引发和微波聚合快速制备分子印迹毛细管电色谱整体柱

以光引发和微波聚合的方式快速制备了以对羟基苯甲酸为模板的分子印迹毛细管电色谱整体柱.实验结果表明,光聚合整体柱材料较微波聚合整体柱材料颗粒大、孔径大、通透性好,两种方法制备的整体柱都显示出了较好的印迹效果,可实现羟基苯甲酸异构体的分离,但由于后者孔径小、通透性差,需采用p-CEC模式.同时,两种方法制备的整体柱还能够实现中性化合物的分离.

Capillary Electrochromatography of Molecularly Imprinted Monolithic Columnvia Photo-initiated and Microwave Polymerization

A 4-hydroxybenzoic acid imprinted column was prepared by in situ photo-initiated and microwave polymerization in a capillary column.The results show that the particle diameter and pore size of monolithic column materials by in situ photo-initiated is larger than microwave irradiation.Meanwhile,the former has good permeability.Good molecular recognition was achieved for p-hydroxybenzoic acid and good resolution of isomers can be realized.In addition,the mode of pressure-driven capillary electrochromatography is needed on the monolithic column by microwave irradiation,caused by small particle diameter and pore size.Furthermore,some neutral compounds could also be baseline-separated on the imprinted polymer columns by photo initiated and microwave irradiation.