Effects of fermentation by lactic acid bacteria on the antigenicity of bovine whey proteins |
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| Authors: | Guanhao Bu Yongkang Luo Ying Zhang Fusheng Chen |
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| Affiliation: | 1. College of Cereal and Food Science, Henan University of Technology, Zhengzhou, P.R. China;2. Functional Dairy Lab, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, P.R. China |
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| Abstract: | BACKGROUND: The main whey proteins α‐lactalbumin (α‐LA) and β‐lactoglobulin (β‐LG) are considered as the major allergens in cow's milk. Microbial fermentation can produce some proteolytic enzymes, which can induce the degradation of milk protein allergens. In this study, the effects of fermentation by lactic acid bacteria on the antigenicity of α‐LA and β‐LG were investigated using indirect competitive ELISA. Meanwhile, the proteolysis of milk proteins was detected by TNBS assay and SDS‐PAGE electrophoresis. RESULTS: Fermentation by lactic acid bacteria could significantly reduce the antigenicity of α‐LA and β‐LG in skim milk. Combined strains of Lactobacillus helveticus and Streptococcus thermophilus were the most effective in reducing the antigenicity of both whey proteins. In addition, α‐LA and β‐LG antigenicity decreased to a lower value at 6 h of fermentation and at 0.5 d of cold storage by fermentation with the combined strains. The results of TNBS assay and SDS‐PAGE electrophoresis showed that lactic acid bacteria strains used in this study hydrolysed whey proteins only to a limited extent. CONCLUSION: The fermentation with lactic acid bacteria is an effective way to reduce whey proteins antigenicity. Copyright © 2010 Society of Chemical Industry |
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| Keywords: | cow's milk allergy whey proteins antigenicity ELISA fermentation lactic acid bacteria |
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