The (193–209) 17‐residues peptide of bovine β‐casein is transported through Caco‐2 monolayer |
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Authors: | Daniela Regazzo Daniel Mollé Gianfranco Gabai Daniel Tomé Didier Dupont Joëlle Leonil Rachel Boutrou |
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Affiliation: | 1. Department of Experimental Veterinary Science, University of Padua, Viale dell'Università, Legnaro, Padua, Italy;2. INRA, UMR1253, Science et Technologie du Lait et de l'?uf, Rennes, France;3. Agrocampus Ouest, UMR1253, Science et Technologie du Lait et de l'?uf, Rennes, France;4. AgroParisTech, CRNH‐IdF, UMR914 Nutrition Physiology and Ingestive Behavior, Paris, France |
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Abstract: | Although the bioavailability of large peptides with biological activity is of great interest, the intestinal transport has been described for peptides up to only nine residues. β‐casein (β‐CN, 193–209) is a long and hydrophobic peptide composed of 17 amino acid residues (molecular mass 1881 Da) with immunomodulatory activity. The present work examined the transport of the β‐CN (193–209) peptide across Caco‐2 cell monolayer. In addition, we evaluated the possible routes of the β‐CN (193–209) peptide transport, using selective inhibitors of the different routes for peptide transfer through the intestinal barrier. The results showed that the β‐CN (193–209) peptide resisted the action of brush‐border membrane peptidases, and that it was transported through the Caco‐2 cell monolayer. The main route involved in transepithelial transport of the β‐CN (193–209) peptide was transcytosis via internalized vesicles, although the paracellular transport via tight‐junctions could not be excluded. Our results demonstrated the transport of an intact long‐chain bioactive peptide in an in vitro model of intestinal epithelium, as an important step to prove the evidence for bioavailability of this peptide. |
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Keywords: | Caco2‐cells Immunomodulatory peptide Intestinal transport Mass spectrometry |
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