| TNPP(四硝基卟啉)测定奶粉中锌的含量 |
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| 引用本文： | 杨会琴,李桂琴,韩士田. TNPP(四硝基卟啉)测定奶粉中锌的含量[J]. 食品科学, 2007, 28(5): 278-280 |
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| 作者姓名： | 杨会琴 李桂琴 韩士田 |
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| 作者单位： | 河北师范大学化学学院； 河北经贸大学生物科学与工程学院； 河北师范大学化学学院 河北石家庄050061河北经贸大学生物科学与工程学院； 河北石家庄050061； |
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| 摘 要： | 本实验研究了锌与非水溶性试剂TNPP(四硝基卟啉)的显色反应。采取分光光度泫,测定了酸碱度、放置时间等多个方面的因素对络合物的生成量及稳定性的影响,确定了反应进行的最适条件,建立测定食品中锌含量的分析方法。实验发现,在盐酸羟胺存在的条件下,TNPP(四硝基卟啉)能与锌在微酸性介质(pH6)中生成淡紫色络合物,该反应在60℃下只需在加热15min即可生成完全,经实验测得,该络合物的最大吸收波长在536nm处,络合比为1∶1,且当锌含量在0～10μg／ml范围内时,测得的曲线呈线性关系,服从比尔定律。并将其用于食品中痕量锌的测定,结果令人满意。
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| 关 键 词： | 锌 TNPP(四硝基卟啉) 分光光度法 |
| 文章编号： | 1002-6630（2007）05-0278-03 |
| 修稿时间： | 2006-06-30 |
Study on System of Color Reaction of Zn with Meso-tetrakis(4-sulfonatophenyl) Porphyrin (TNPP) and Its Application in Some Food Analysis |
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| YANG Hui-qin,LI Gui-qin,HAN Shi-tian. Study on System of Color Reaction of Zn with Meso-tetrakis(4-sulfonatophenyl) Porphyrin (TNPP) and Its Application in Some Food Analysis[J]. Food Science, 2007, 28(5): 278-280 |
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| Authors: | YANG Hui-qin LI Gui-qin HAN Shi-tian |
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| Affiliation: | 1.College of Chemistry,Hebei Normal University,Shijiazhuang 050061,China; 2.College of Biology Science and Engineering,Hebei Economy and Trade University,Shijiazhuang 050061,China |
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| Abstract: | The color reaction of Zn and TNPP(meso-tetrakis(4-sulfonatophenyl)porphyrin)was studied in this paper,with spectrophotometric method,to assay the effects on pH,standing time and stability of this complex.An excellent method to be used to determine Zn in foods has been ascertained.The experimental results indicated that in the presence of hydroxylamine hydrochloride,with ortho-dinitrogenous ptenanthrene,and Seignette salt as masking agents,TNPP and Zn present light purple in an alkaline solution of pH6,at room temperature.Beer’s law is obeyed in the range of 0～10μg/mi of Zn.This method has been used to determine micro amounts of Zn in foods with satisfactory results. |
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| Keywords: | Zn TNPP spectrophotometric method |
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