黑曲霉发酵豆粕产蛋白酶活性的研究

采用黑曲霉AS3.350发酵豆粕,研究其所产的蛋白酶的活力。运用响应面分析法优化影响发酵的主要因素:接种量、初始pH值、发酵培养基浓度等,采用多元二次回归方程拟和上述3因素与蛋白酶活力间的函数关系,确定了黑曲霉发酵豆粕产蛋白酶的最佳发酵条件,为黑曲霉发酵豆粕产大豆肽的研究提供了相应的工艺参数和一定的理论依据,有利于提高大豆肽的产率。

Study on protease production by Aspergillus niger on soybean meal

The ability of Aspergillus niger AS 3.350 for the production of protease on soybean meal was studied.By response surface method(RSM),the main fermentation factors were optimized,including inoculum,initial pH and the concentration of fermentation medium.Using multi-quadratic regression equation to fit the functional relationships between three above factors and protease activity,the optimal fermentation conditions for the production of protease by A.niger 3350 on soybean meal were determined.The results could be used as technical parameters and theoretic basis for the production of soybean peptides with A.niger on soybean meal,which were helpful for increasing the production rate of soybean peptides.