远端缺血再灌注预处理对心肌细胞膜ATP敏感性钾离子通道表达的影响

目的: 观察远端肢体缺血再灌注预处理后心肌细胞膜K_ATP通道在不同时点的表达变化。方法: 夹闭大鼠双侧股动脉 5 min 后开放 5 min,如此重复3次完成远端肢体缺血再灌注预处理,分别于处理后1、4、24和48 h处死大鼠,取其左心室肌组织,检测其心肌细胞膜K_ATP通道各亚基表达变化。结果: 远端肢体缺血再灌注预处理后,心肌细胞膜K_ATP通道的表达量增加,在4～24 h内增加最明显。结论: 远端肢体缺血再灌注预处理可上调心肌细胞膜K_ATP通道的表达,这种表达的改变可能参与了远端肢体缺血再灌注预处理对心脏的保护作用机制。

Effect of remote ischemic preconditioning on the expression of myocardial ATP-sensitive potassium channel

AIM: To investigate the expression of sarcolemmal ATP-sensitive potassium channel in cardiomyocytes after remote ischemic preconditioning (RIPC).METHODS: Rat models of remote ischemic preconditioning were established by clipping bilateral femoral artery 5 minutes and opening 5 minutes for 3 cycles. Then, the expression of K_ATP channel in cardiomyocytes was evaluated in control group and groups at 1h, 4h, 24h and 48h postoperatively.RESULTS: The relative protein expression level of sarcolemmal ATP-sensitive potassium channel in cardiomyocytes was increased after RIPC.CONCLUSION: The expression of sarcolemmal ATP-sensitive potassium channel was increased after RIPC, the change of expression in ATP-sensitive potassium channel may play a role in the cardiac protection of RIPC against ischemia.