芩柏复方烫伤膏对大鼠实验性烫伤模型的保护作用

目的: 研究芩柏复方烫伤膏对大鼠深Ⅱ度烫伤模型烫伤创面的保护作用。方法: SD大鼠100只,建立大鼠深Ⅱ度烫伤模型,随机分为5组:模型对照组,芩柏复方烫伤膏组(0.4,0.2,0.1 g 浸膏/g生药),湿润烧伤膏组(0.32 g 浸膏/g生药),创面外用药物,每天一次,连续 21 d。在伤后不同时间点取材,测定药物对烫伤创面的结痂面积和脱痂愈合时间、病理组织学和微血管密度(MVD)的影响;测定药物对烫伤大鼠血清丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性的影响。结果: 芩柏复方烫伤膏(0.4,0.2,0.1 g 浸膏/g生药)在不同时相点可不同程度地减少大鼠烫伤创面结痂面积,明显缩短脱痂愈合时间;改善烫伤创面病理组织学变化;升高烫伤创面MVD含量;降低烫伤大鼠血清升高的MDA含量,升高SOD水平。结论: 芩柏复方烫伤膏对大鼠深Ⅱ度烫伤具有良好的保护作用,能明显促进大鼠深Ⅱ度烫伤创面愈合,改善创面病理组织学的变化,其机制可能与促进创面微血管生成,降低烫伤大鼠血清MDA含量,提高SOD活力,抗脂质过氧化等有关。

Protective effect of QBFFTSG on the wound healing of skin burns in rats

AIM: To investigage the effects of Qin bai fu fang tang shang gao (QBFFTSG) on burn wound healing in rats.METHODS: The model of deep grade Ⅱskin burn wounds in rats was established. A total of 130 model rats were divided into 5 groups as follows: high, middle and low concentration of QBFFTSG treatment group, non - treatment group, Shi run shao shang gao (SRSSG) treatment group was used as positive control. Scab area, healing time of burn wound and wound tissues were taken at different times after wound induction for analysis and assessment of each parameter. Histopathology of wound tissues and microvascular density (MVD) with factor Ⅷ immunohistochemistry staining were measured. The content of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) were determined.RESULTS: QBFFTSG (0.4, 0.2, 0.1 g extract/g crude drug) could decrease the scab area and healing time, improve the histopathological changes of burn wound, increase the MVD density, decrease the elevated serum MDA content and increase the serum SOD level in rats at varying degrees during wound healing.CONCLUSION: QBFFTSG produces an enhanced protective effect on wound tissues of rats which could obviously promote the wound healing and improve the histopathological changes of wounds. The mechanism of action of QBFFTSG may be attributed to wound angiogenesis and anti-lipid peroxidation in rats.