玉米芯果胶多糖纯化及组分鉴定

为提高玉米芯经济利用价值，采用DEAE-Cellulose52柱层析法纯化粗制玉米芯果胶多糖，并解析其糖成分. 研究表明，适宜的阴离子柱层析条件为6 mL-4%-1.5 mL/min-4∶2（VdH2O∶VNaCl），得率可提高一倍以上，且产品为高纯度玉米芯果胶多糖. 其多糖成分由D-半乳糖醛酸（D-Galacturonic acid，D-GalA）、木糖（Xylose，Xyl）和鼠李糖（Rhamnose，Rha）构成，其中D-GalA达到49.23%，为主要成分；三糖含量合计为61.66%，为杂多糖. 玉米芯果胶多糖可应用于食品工业，也可开发出新型功能食品.

Purification of Corncob Pectin and Identification of Its Composition

In order to increase corncob’s economic availability，crude corncob pectin is purified by DEAE-Cellulose52 and its sugar composition is analyzed. The results show that optimum column conditions are 6 mL-4%-1.5 mL/min-4∶2（VdH2O∶VNaCl）for purifying corncob pectin. At this conditions，purified pectin yield is increased twice or more with highly pure. Corncob pectin contains three sugar composition at least，such as D-galacturonic acid（D-GalA），xylose and rhamnose，so corncob pectin belongs to heteropolysaccharide. D-GalA is a major composition，about 49.23% in purified pectin；while the content of three is 61.66%. Summing up，this corncob pectin can be used in food industry，and also in developing new functional foods for human.