不同提取方法对金华火腿粗肽液抗氧化活性的影响

研究两种提取方法(磷酸盐和盐酸)所得金华火腿粗肽液的抗氧化活性,以自由基的清除能力,金属离子螯合能力,还原力以及总抗氧化能力为测定指标,以还原型谷胱甘肽(GSH)作对照。结果表明:磷酸盐法提取的金华火腿粗肽液(P)多肽含量显著(p<0.05)高于盐酸法提取的金华火腿粗肽液(H);质量浓度低于5mg/m L时,P和H清除DPPH自由基与超氧阴离子自由基能力无显著差异;P螯合金属离子的能力显著(p<0.05)高于H和GSH;当质量浓度为4mg/m L时,P还原力显著(p<0.05)高于H;当质量浓度为1mg/m L时,P总抗氧化能力达到GSH的48%,显著高于H(p<0.05)。因此磷酸盐所提取的金华火腿粗肽液抗氧化能力优于盐酸所提取的金华火腿粗肽液。 

Effect of extraction methods on the antioxidant activity of crude peptides from Jinhua ham

Crude peptides were extracted by two solutions from Jinhua ham and evaluated for antioxidant activity by radical scavenging,metal ion chelating,reducing power and the total antioxidant capacity(T-AOC) with GSH as control. The results showed that the crude peptide extract from Jinhua ham using PBS as solution(crude peptide P) presented higher peptide content than crude peptide extract using HCl as solution(crude peptide H). The DPPH radical scavenging capacity and the superoxide anion radical scavenging activities of crude peptide P did not differ significantly from that of crude peptide H below 5mg/m L. In addition,the crude peptide P showed higher metal ion chelating activity than crude peptide H and GSH,which presented higher reducing power than crude peptide H at 4mg/m L(p <0.05). Total antioxidant capacity of crude peptide P reached 48% of GSH at 1mg/m L,which was significantly higher than crude peptide H(p<0.05). In conclusion,crude peptide P had higher antioxidant activity than crude peptide H.