火炭母总黄酮的提取和抗氧化活性研究

对火炭母总黄酮的提取工艺及抗氧化活性进行了研究。在单因素实验的基础上考察了料液比、乙醇浓度、提取时间、提取温度对总黄酮提取的影响,再通过正交实验确定提取的最佳工艺为:料液比1∶50g/m L、乙醇浓度50%、提取温度70℃、提取时间20min。在此条件下,火炭母总黄酮的提取率为4.28%。抗氧化结果表明,火炭母黄酮具有较强的还原能力,对DPPH·和OH·两种自由基具有明显的清除能力,且在实验浓度范围内清除率与浓度呈现出良好的量效关系,半数有效浓度EC50分别为0.00519、0.150mg/m L。 

Study on extraction and antioxidant activity of total flavonids from Polygonum chinense

The ultrasonic extraction process and antioxidant activity of total flavonids from Polygonum chinense were studied. The effects of four factors on extraction rate for total flavonoids were discussed by single factor experiment including material-liquid ratio,ethanol concentration,extraction time and extraction temperature.The orthogonal experiment showed the optimum conditions were as follows :material-liquid ratio 1 ∶50g/m L,ethanol concentration 50%,extraction temperature 70℃,extraction time 20 min. Under these conditions,the extraction rate of total flavonoids was 4.28%. The results of antioxidation showed that the flavonoids from Polygonum chinense had good reduction ability,and also had significant scavenging activity on DPPH· and OH·,the scavenging rates to free radicals were positive proportional to the flavonoids concentration. The EC50 of scavenging activity to DPPH· was 0.00519mg/m L. The EC50 of scavenging activity to OH· was 0.150mg/m L.