大气压电离液质联机的应用

本文以我们几年来的实际工作为素材,对电喷雾（ESI）和大气压化学电离（APCI）这一近年发展起来的软电离质谱接口技术做了应用性介绍。文中列举了该技术用于药物代谢、蛋白质构象以及热不稳定化合物分析的几个实例,对一些典型化合物在离子源内经碰撞诱导解离（CID）形成的质谱开裂产物进行了解析,对流量匹配、脱溶剂的技术特点、离子在溶液中的预形成、溶剂的选择、仪器的噪声等实践中遇到的问题做了较为详细的讨论。

Application of LC/MS with ESI/APCI Interface

This paper is an introhuction for the usage of LC/MS with ESI(air pressure ionizition)and APCI (air pressure chemical ionization) as interface. All the material is from our work on LC/MS and doping test during the recent years. As example the work on drug metabolism, conformational change of protein in solution as well as some analysis for labile compounds are presented in the paper and some practical problerns are discussed.