| 栀子甾醇的分离分析 |
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| 引用本文： | 甘辉,万明,罗阳帆,陈勇辉,付桂明,万茵.栀子甾醇的分离分析[J].江西食品工业,2010(4):35-37. |
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| 作者姓名： | 甘辉 万明 罗阳帆 陈勇辉 付桂明 万茵 |
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| 作者单位： | [1]江西省南昌市东湖区卫生防疫站,330000 [2]南昌县莲塘镇农业服务中心,330200 [3]南昌大学生命科学与食品工程学院,330047 |
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| 摘 要： | 本文对栀子的植物甾醇成分进行分离分析,以期扩大栀子的应用范围。文中用索氏提取法从栀子中提取出果油,得率为16.7%。通过皂化法,用乙醚萃取出果油中的粗甾醇,再以溶剂结晶法对粗甾醇进行纯化。最后对所得甾醇样品的组分采用HPLC-MS分析。结果表明,栀子果油中可能含有β-谷甾醇和甘氨胆酸等甾醇成分。
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| 关 键 词： | 栀子 植物甾醇 HPLC-MS |
Extraction and component analysis of phytosterol in Gardenia |
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| Gan Hui,Wan Ming,Luo Yang-fan,Chen Yong-hui,Fu Gui-ming,Wan Yin.Extraction and component analysis of phytosterol in Gardenia[J].Jiangxi Food Industry,2010(4):35-37. |
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| Authors: | Gan Hui Wan Ming Luo Yang-fan Chen Yong-hui Fu Gui-ming Wan Yin |
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| Affiliation: | 1 Health and Epidemic prevention Station of East Lake District, Nanchang City, Jiangxi Province, 330008; 2 Liantang Town Agricultural Services Center, Nanchang City, Jiangxi Province, 330200; 3 Life science and food thchnology college of Nanchang University, Nanchang City, Jiangxi Province, 330047) |
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| Abstract: | This paper was about the extraction and component analysis of phytosterol in Gardennia. The oil in the Gardennia was extracted by soxhlet extraction and the yield was 16.7%. Then the oil was saponified by ethanolic KOH, and the phytosterol in non-saponificable fraction was extracted with diethylether. Solvent crystallization was used to edulcorate phytosterol. Then the phytosterol was analyzed by HPLC-MS. The result showed that the component of Gardennia phytosterol maybe involved β-sitosterol and glycocholic acid. |
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| Keywords: | Gardenia phytosterol HPLC-MS |
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