| 转基因番茄表达口服乙肝疫苗 |
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| 引用本文： | 郝浩永,尉亚辉,朱剑光,孙杰,王娅宁,荆二勇,张变玲,薛柯.转基因番茄表达口服乙肝疫苗[J].食品科学,2007,28(6):201-204. |
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| 作者姓名： | 郝浩永 尉亚辉 朱剑光 孙杰 王娅宁 荆二勇 张变玲 薛柯 |
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| 作者单位： | 西北大学西部资源生物与现代生物技术教育部重点实验室； 西北大学西部资源生物与现代生物技术教育部重点实验室 陕西西安710069； 陕西西安710069； |
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| 摘 要： | 将构建好的植物表达载体pCAMBIA1301/HB转化到根癌农杆菌LBA4404中,并通过农杆菌介导转化番茄。所得再生植株经PCR和Southern杂交鉴定确认成功整合了HBsAg(hepatitis B surface antigen)基因,SDS-PAGE显示蛋白表达与非转化植株相比有明显差异,ELISA检测和Western blotting证实HBsAg已在转化植株中表达,分子量约为24kD。
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| 关 键 词： | 口服疫苗 乙型肝炎表面抗原 番茄 转基因植物 |
| 文章编号： | 1002-6630（2007）06-0201-04 |
| 修稿时间： | 2006-08-07 |
Expression of Oral Hepatitis B Vaccine in Transgenic Tomato |
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| HAO Hao-yong,WEI Ya-hui,ZHU Jian-guang,SUN Jie,WANG Ya-ning,JING Er-yong,ZHANG Bian-ling,XUE Ke.Expression of Oral Hepatitis B Vaccine in Transgenic Tomato[J].Food Science,2007,28(6):201-204. |
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| Authors: | HAO Hao-yong WEI Ya-hui ZHU Jian-guang SUN Jie WANG Ya-ning JING Er-yong ZHANG Bian-ling XUE Ke |
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| Affiliation: | Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi’an 710069, China |
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| Abstract: | Constructed plant expressive vector pCAMBIA1301/HB was changed intoAgrobacterium tumefaciens LBA4404 by which tomato was transformed. By PCR and Southern blotting, regenerated plants were validated that H-BsAg gene is transformed successfully. Notable distinction of protein between transformed and untransformed plants was observed by SDSPAGE. ELISA and Western blotting indicated H-BsAg has expressed in transformed plants,with MW about 24 kD. |
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| Keywords: | oral vaccine HBsAg tomato transgenic plant |
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