Separation of EPA and DHA in fish oil by lipase-catalyzed esterification with glycerol |
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Authors: | Arnar?Halldorsson Bjorn?Kristinsson Caroline?Glynn Email author" target="_blank">Gudmundur?G?HaraldssonEmail author |
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Affiliation: | (1) Science Institute, University of Iceland, Dunhaga, 107 Reykjavik, Iceland |
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Abstract: | The objective of this study was to investigate the use of lipases as catalysts for separating EPA and DHA in fish oil by kinetic
resolution based on their FA selectivity. Esterification of FFA from various types of fish oils with glycerol by immobilized
Rhizomucor miehei lipase under water-deficient, solvent-free conditions resulted in a highly efficient separation of EPA and DHA. Reactions
were conducted at 40°C with a 10% dosage of the lipase preparation under vacuum to remove the coproduced water, thus rapidly
shifting the reaction toward the products. The bulk of the FA, together with EPA, were converted into acylglycerols, whereas
DHA remained in the residual FFA. As an example, when FFA from tuna oil comprising 5% EPA and 25% DHA were esterified with
glycerol, 90% conversion into acylglycerols was obtained after 48 h. The residual FFA contained 78% DHA and only 3% EPA, in
79% DHA recovery. EPA recovery in the acylglycerol fraction was 91%. The type of fish oil and extent of conversion were highly
important parameters in controlling the degree of concentration. |
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Keywords: | DHA EPA esterification n− 3 fatty acids fish oil glycerol kinetic resolution lipase Lipozyme RM IM Rhizomucor miehei lipase |
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