Protective Effect of N-Acetylserotonin against Acute Hepatic Ischemia-Reperfusion Injury in Mice |
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| Authors: | Shuna Yu Jie Zheng Zhengchen Jiang Caixing Shi Jin Li Xiaodong Du Hailiang Wang Jiying Jiang Xin Wang |
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| Affiliation: | 1.Departments of Anatomy, Weifang Medical University, Weifang 261053, China; E-Mails: (S.Y.); (Z.J.); (C.S.); (J.L.); (X.D.); (H.W.);2.Departments of Pathology, Weifang Medical University, Weifang 261053, China; E-Mail: ;3.Department of Neurosurgery, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA 02115, USA |
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| Abstract: | The purpose of this study was to investigate the possible protective effect of N-acetylserotonin (NAS) against acute hepatic ischemia-reperfusion (I/R) injury in mice. Adult male mice were randomly divided into three groups: sham, I/R, and I/R + NAS. The hepatic I/R injury model was generated by clamping the hepatic artery, portal vein, and common bile duct with a microvascular bulldog clamp for 30 min, and then removing the clamp and allowing reperfusion for 6 h. Morphologic changes and hepatocyte apoptosis were evaluated by hematoxylin-eosin (HE) and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining, respectively. Activated caspase-3 expression was evaluated by immunohistochemistry and Western blot. The activation of aspartate aminotransferase (AST), malondialdehyde (MDA), and superoxide dismutase (SOD) was evaluated by enzyme-linked immunosorbent assay (ELISA). The data show that NAS rescued hepatocyte morphological damage and dysfunction, decreased the number of apoptotic hepatocytes, and reduced caspase-3 activation. Our work demonstrates that NAS ameliorates hepatic IR injury. |
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| Keywords: | N-acetylserotonin newborn mouse hepatic ischemia-reperfusion injury apoptosis |
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