Thiobacillus denitrificans immobilized biotrickling filter for NO2 removal

Nitrogen dioxide (NO₂) removal efficiency of a biotrikling filter was evaluated under different operating conditions. Activated alumina (AA) was used as the immobilization matrix for Thiobacillus denitrificans (T. denitrificans) in the biotrickling filter. Batch studies were conducted to find out the degradation kinetics of nitrate and nitrite for a concentration range of 600–10,000 mg/L expressed as nitrogen. Nitrite exhibited maximum degradation rate followed by nitrate. Electron acceptor in the form of NO₂ gas showed least removal efficiency. Bio-kinetic parameters for T. denitrificans, by utilizing nitrate and nitrite as electron acceptors, were also evaluated. The μ_max (Maximum specific growth rate) and Y_T (Yield coefficient) values for T. denitrificans in the presence of nitrate and nitrite were 1.03 h⁻¹, 0.275 and 0.63 h⁻¹, 0.1316 respectively. Column study was conducted to find the adsorption and desorption potential of activated alumina. The adsorbed NO₂ from AA could easily be desorbed using distilled water with an efficiency of 76±0.8%. Once fed batch studies were conducted to evaluate the NO₂ removal efficiency by a biotrickling filter. With an influent NO₂ gas concentration of 2,735 ppm, the reactor could achieve a removal efficiency of 99% within 2 min from gas phase and within 96 h from the liquid phase, with an average biomass concentration of 200 mg/g of AA. The mechanism of NO₂ gas removal in the biotrickling filter seems to be the dissolution of NO₂ in water to form NO₃⁻, conversion of NO₃⁻ to NO₂ ⁻, and finally to N₂ gas.