Immobilization of iron storage protein on a gold electrode based on self-assembled monolayers |
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Authors: | Won Keehoon Park Mi Jin Yoon Hyon Hee Kim Ji Hyeon |
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Affiliation: | Department of Chemical and Biochemical Engineering, Dongguk University, Seoul 100-715, Republic of Korea. |
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Abstract: | Ferritin is a globular protein consisting of 24 subunits to form a hollow shell and is capable of storing iron in the cavity. Findings that the naturally existing iron core of ferritin can be readily extracted and replaced with a variety of electroactive materials make ferritin suitable for biosensor and biofuel cell applications. The immobilization of ferritin on the electrode surface is essential for various bioelectronic applications. In this work, based on self-assembled monolayers, ferritin was immobilized on a gold electrode through two different methods: chemisorption of thiolated ferritin onto bare gold electrodes and covalent binding of ferritin to succinimidyl alkanedisulfide-modified Au electrodes. Effects of experimental conditions on the ferritin immobilization were investigated. The ferritin immobilized on the gold electrode was characterized by atomic force microscopy and cyclic voltammetry. |
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Keywords: | Ferritin immobilization Atomic force microscopy Cyclic voltammetry Self-assembled monolayers |
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