高效液相色谱法检测粮油中黄曲霉毒素方法验证

该文报道验证同时检测粮食、植物油中黄曲霉毒素B1、B2、G1和G2的免疫亲和柱净化-柱后光化学衍生-高效液相色谱方法.样品经甲醇-水(体积比为70∶30)提取,通过免疫亲和柱富集和净化,采用Cloversil C18色谱柱(4.6mm×150 mm,粒度5μm),以甲醇:水(50∶50)溶液为流动相,柱后光化学衍生,利...

Method validation on determination of aflatoxins in cereal and oil by high performance liquid chromatography

This paper verifies for the simultaneous determination of aflatoxins(AFs)Bi,B2,Gi andG2 in cereals and oil by high performance liquid chromatography(HPLC) with fluorescence detection after immunoaffinity column clean-up and post-column derivatization,cereals and oil sample was extracted with methanol-water(70:30,v/v).The extract was purified by immunoaffinity column and the aflatoxins were separated by reversed-phase HPLC,and quantified with fluorescence detection after photochemical derivatization.The separation was performed on a cloversil column(4.6mm i.d.×150 mm,5 urn) with a mobile phase consists of methanol-water(70/30,v/v),quantified with fluorescence detection after photochemical derivatization.The limits of detection were 0.5μg,0.2μg,0.7μg,0.2μg/kg for AFs B_1,B_2,G_1 and G_2.The calibration curves for AFs B_1,B_2,G_1 and G_2 were made in the concentration range:0.51～10.19ng/mL,0.15～2.92 ng/mL,0.54～10.71ng/mL,0.16～3.3ng/mL, the cereals and oil sample recoveries were from 70.3%～109.8%,relative standard deviations were between 1.4%and 9.1%.