| 60Coγ射线对胃癌细胞STAT3表达的影响研究 |
| |
| 引用本文： | 高玲,李峰生,陈肖华,刘青杰. 60Coγ射线对胃癌细胞STAT3表达的影响研究[J]. 辐射研究与辐射工艺学报, 2011, 29(2): 113-116 |
| |
| 作者姓名： | 高玲 李峰生 陈肖华 刘青杰 |
| |
| 作者单位： | 中国疾病预防控制中心辐射防护与核医学安全所;军事医学科学院放射与辐射医学研究所;第二炮兵总医院; |
| |
| 基金项目： | 国家自然科学基金项目(30870749、30570551)资助 |
| |
| 摘 要： | 为了探讨电离辐射对胃腺癌细胞中STAT3活性及定位的影响,首先利用Western Blot方法检测60Coγ射线照射后胃癌BGC-823细胞内STAT3蛋白含量及活性变化,随后提取60Coγ辐射前后BGC-823细胞核蛋白,检测细胞核内STAT3水平,最后利用免疫荧光染色进一步验证辐射前后STAT3在BGC-823细胞...
|
| 关 键 词： | 胃癌细胞 电离辐射 STAT3 入核 |
Effects of ionizing radiation on the STAT3 expression of gastric adenocarcinoma cells |
| |
| GAO Ling,LI Fengsheng,CHEN Xiaohua,LIU Qingjie. Effects of ionizing radiation on the STAT3 expression of gastric adenocarcinoma cells[J]. Journal of Radiation Research and Radiation Processing, 2011, 29(2): 113-116 |
| |
| Authors: | GAO Ling LI Fengsheng CHEN Xiaohua LIU Qingjie |
| |
| Affiliation: | GAO Ling1,2 LI Fengsheng3 CHEN Xiaohua2 LIU Qingjie11(National Institute for Radiological Protection,China CDC,Beijing 100088,China)2(Institution of Radiation Medicine,Academy of Military Medical Sciences,Beijing 100850,China)3(General Hospital of Second Artillery,China) |
| |
| Abstract: | This study is to investigate the effect of ionizing radiation on the activity and localization of STAT3 in gastric adenocarcinoma cells.After irradiated with 60Co γ-rays,the content and activity of STAT3 in gastric adenocar-cinoma BGC-823 cells were detected by Western blot.The content of STAT3 in nucleus of BGC-823 was further de-tected by Western blot after the total protein in nucleus of irradiated BGC-823 was extracted.Finally,the distribution of p-STAT3（Y705） in BGC-823 treated with or without irradiation was examined by immunofluorescence staining.The results showed that the level of p-STAT3（Y705） in BGC-823 cells decreased significantly after 60Co γ-rays irradiation,and the content of STAT3 in BGC-823 cells nuclei also decreased after irradiation.As predicted,the p-STAT3（Y705） localized in nucleus of BGC-823 cells also decreased significantly.60Co γ-rays irradiation inhibited the phosphoryla-tion of p-STAT3（Y705） and further suppress the nucleus localization of STAT3,which might be involved in radia-tion-induced cell proliferation inhibition and apoptosis of gastric adenocarcinoma cells. |
| |
| Keywords: | Gastric adenocarcinoma cell Ionizing radiation STAT3 Nucleus localization |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
