| 耐辐射球菌PprI蛋白质表达及其纯化的实验研究 |
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| 引用本文： | 张永芹,周辉,陈洁,杨占山.耐辐射球菌PprI蛋白质表达及其纯化的实验研究[J].辐射研究与辐射工艺学报,2011,29(2):117-122. |
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| 作者姓名： | 张永芹 周辉 陈洁 杨占山 |
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| 作者单位： | 苏州大学医学部放射医学与公共卫生学院; |
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| 基金项目： | 国防基础科研项目(A3820060138); 国家自然科学基金项目(30570549)资助 |
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| 摘 要： | 为了在大肠杆菌中高效表达耐辐射球菌PprI融合蛋白并进行纯化,本研究以PCMV-HA-pprI质粒为模板,PCR扩增pprI基因全序列,经NcoI和EcoRI双酶切后定向克隆到pET-28a表达载体中,构建重组原核表达质粒pET-28a-His-pprI,并转入E.coli BL21(DE3)RP.IPTG诱导融合蛋白...
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| 关 键 词： | 耐辐射球菌 pprI基因 原核表达质粒 蛋白表达 纯化 |
Expression and purification of PprI protein from D.radiodurans R1 in escherichia coli |
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| ZHANG Yongqin,ZHOU Hui,CHEN Jie,YANG Zhanshan.Expression and purification of PprI protein from D.radiodurans R1 in escherichia coli[J].Journal of Radiation Research and Radiation Processing,2011,29(2):117-122. |
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| Authors: | ZHANG Yongqin ZHOU Hui CHEN Jie YANG Zhanshan |
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| Affiliation: | ZHANG Yongqin ZHOU Hui CHEN Jie YANG Zhanshan(School of Radiation Medicine and Public Health,Soochow University,Suzhou 215213,China) |
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| Abstract: | In order to express and purify PprI protein from D.radiodurans R1 in E.coli,the full length of pprI gene was gained by PCR amplification using pCMV-HA-pprI as a template.The gene segment was inserted into vec-tor pET-28a after digested by two restriction endonucleases Nco I and EcoR I.Then the recombinant vector pET-28a-His-pprI was transfected into E.coli BL21(DE3) RP.The PprI protein expression was induced by IPTG and the fusion protein was confirmed by SDS-PAGE and Western blotting.The expressive conditi... |
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| Keywords: | D radiodurans R1 PprI gene Prokaryotic recombinant vector Protein expression Protein purification |
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