| 乙型肝炎核心抗体放射免疫定量试剂盒的研制 |
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| 引用本文： | 官国英,孔繁信,王衍真,刘一兵,隗秀芳,王云艳. 乙型肝炎核心抗体放射免疫定量试剂盒的研制[J]. 同位素, 2004, 17(3): 143-146 |
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| 作者姓名： | 官国英 孔繁信 王衍真 刘一兵 隗秀芳 王云艳 |
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| 作者单位： | 中国原子能科学研究院同位素研究所,北京,102413 |
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| 摘 要： | 以聚苯乙烯小球为固相载体,在小球表面包被一层基因工程制备的乙型肝炎核心抗原(HBcAg),以125I标记的乙型肝炎核心抗体(125I-Anti-HBc)作为标记物,采用固相放射免疫竞争法研制成乙型肝炎核心抗体放射免疫定量试剂盒(Anti-HBc-RIA)。本试剂盒的灵敏度为0.8NCU/mL,批内变异系数<9.9%,批间变异系数<13.8%,回收率为92.6%～109.2%。正常参考值为0～2.0NCU/mL。
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| 关 键 词： | 乙型肝炎病毒（HBV） 乙型肝炎核心抗体（Anti-HBc） 固相放射免疫分析 |
| 文章编号： | 1000-7512(2004)03-0143-04 |
| 修稿时间： | 2003-12-23 |
The Establishment of Solid Phase Radioimmunoassay for Quantitative Determination of Hepatitis B Core Antibody |
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| GUAN Guo-ying,KONG Fan-xin,WANG Yan-zhen,LIU Yi-bing,WEI Xiu-fang,WANG Yun-yan. The Establishment of Solid Phase Radioimmunoassay for Quantitative Determination of Hepatitis B Core Antibody[J]. Isotopes, 2004, 17(3): 143-146 |
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| Authors: | GUAN Guo-ying KONG Fan-xin WANG Yan-zhen LIU Yi-bing WEI Xiu-fang WANG Yun-yan |
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| Abstract: | A sensitive and specific solid phase radioimmunoassay for hepatitis B core antibody(anti-HBc-RIA) is established. Hepatitis B core antigen(HBcAg) is coated on the solid phase beads, ~(125)I-anti-HBc is used as the radioactive tracer. The sensitivity of the assay is 0.8 NCU/mL. The intra-assay CVs and the intre-assay CVs of 3 samples are 9.9% and 13.8% respectively. The analytical recoveries are ranged from 92.6% to 109.2%. The level of 120 normal subjects is 1.2 NCU/mL with a rang of 0~2.0 NCU/mL. The method for measuring anti-HBc in human serum is rapid, sensitive, convenient and inexpensive. It is suitable for clinical and research application. |
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| Keywords: | hepatitis B virus(HBV) hepatitis B core antibody(anti-HBc) solid phase radioimmunoassay |
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