| 高效液相色谱法测定大豆提取物中大豆异黄酮的含量 |
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| 引用本文： | 鞠兴荣,袁建,汪海峰. 高效液相色谱法测定大豆提取物中大豆异黄酮的含量[J]. 中国粮油学报, 2000, 15(4): 26-29 |
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| 作者姓名： | 鞠兴荣 袁建 汪海峰 |
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| 作者单位： | 南京经济学院食品科学与工程系,南京,210003 |
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| 基金项目： | 江苏省教委自然科学基金 |
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| 摘 要： | 本文建立了大豆提取物中大豆异黄酮的高效液相色谱测定方法，通过正交试验了样品水解最佳条件为１．０ｍｏｌ／ｌ ＨＣｌ－ＭｅＯＨ溶解，８０℃下，回流水解０．５ｈ，采用Ｎｏｖａ－Ｐａｋ Ｃ１８ ３．９＊１０ｍｍ４μｍ色谱柱ＭｅＯＨ－０．４％Ｈ３ＰＯ４（４７：５３Ｖ／Ｖ）为流动相，流速０．７ｍｌ／ｍｉｎ检测波长２６０ｎｍ等色谱条件下测定甙元含量，通过换算因子计算大豆异黄酮的含量。该方法快速，灵敏、重理性好，
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| 关 键 词： | 大豆提取物 大豆异黄酮 酸水解 高效液相色谱法 |
Determination of Isoflavone in Extract of Soybean by HPLC |
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| Ju Xinrong,Yuanjian,Wang Haifeng. Determination of Isoflavone in Extract of Soybean by HPLC[J]. Journal of the Chinese Cereals and Oils Association, 2000, 15(4): 26-29 |
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| Authors: | Ju Xinrong Yuanjian Wang Haifeng |
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| Abstract: | A method for determination of isoflavone in extract of soybean by highperformance liquid chromatography(HPLC)is described.The optimum hydrolysis condition of extract of soybean was obtained by using the orthogonal design method and the analysis of variance,the analytical condition of HPLC was also established.The method is rapid,sensitive,reproduciable and provides a recovery of more than 99% with RSD less than 3%. |
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| Keywords: | extract of soybean isoflavone orthogonal design analysis of variance acid-hydrolysis HPLC |
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