| β-葡萄糖苷酶产生菌原生质体的诱变研究 |
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| 引用本文： | 张玲,张芬琦,姚卫蓉.β-葡萄糖苷酶产生菌原生质体的诱变研究[J].食品研究与开发,2006,27(10):62-64. |
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| 作者姓名： | 张玲 张芬琦 姚卫蓉 |
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| 作者单位： | 江南大学食品学院,江苏,无锡,214036 |
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| 摘 要： | 以β-葡萄糖苷酶产生菌——无花果曲霉CN67为原始菌株，经溶菌酶、蜗牛酶和纤维素酶的混合酶系酶解获得原生质体，用紫外线照射诱变，获得产β-葡萄糖苷酶活相对较高的菌株UV-29，其酶活稳定在20．48IU／mL，比出发菌株提高了3．49IU／mL。再经传代实验，该菌株具有较好的遗传稳定性。
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| 关 键 词： | 无花果曲霉 β-葡萄糖苷酶 原生质体诱变 |
| 收稿时间： | 2006-05-22 |
| 修稿时间： | 2006年5月22日 |
STUDY ON MUTAGENESIS OF PROTOPLASTS OF β -GLUCOSIDASE PRODUCING STRAIN |
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| ZHANG Ling,ZHANG Fen-qi,YAO Wei-rong.STUDY ON MUTAGENESIS OF PROTOPLASTS OF β -GLUCOSIDASE PRODUCING STRAIN[J].Food Research and Developent,2006,27(10):62-64. |
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| Authors: | ZHANG Ling ZHANG Fen-qi YAO Wei-rong |
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| Affiliation: | School of Food Science and Technology, Southern Yangtze University,Wuxi 214036, Jiangsu, China |
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| Abstract: | The protoplasts of Aspergillusficuum CN67 was obtained by dissolving it with lywallzyme,snail digestase,cellulose.After UV mutagenization ,the enzymatic ability of the strain is increased from the initial 15.09 IU/ mL to 20.48 IU/mL. And also this mutant has good stability. |
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