超高效液相色谱-质谱应用于违禁药物的检测

气相色谱-质谱是一种能够对药物及其代谢物进行分离和鉴定的方法,然而该方法需要非常困难、繁琐的衍生化和清洗过程。超高效液相色谱-质谱 （UHPLC-MS） 联用技术突破了以上局限并实现了更加快速的分析效果。本文讨论了如何通过对UHPLC-MS方法中固定相、流动相以及检测器方面的优化,在12 min的分离时间内实现了14种违禁药物及其代谢物在pg级水平上的定量。这些违禁药物及其代谢物使用Hypersil GOLD PFP （perfluorophenyl）色谱柱（1.9 μm, 100 mm×2.1 mm）进行分离,并采用单四极杆质谱快速扫描检测。

Use of UHPLC-MS to Determine Illicit Drugs

GC-MS is the established method for the separation and identification of drugs and metabolites; however, it requires difficult and lengthy derivatization and cleanup. Ultrahigh-performance liquid chromatography-mass -spectrometry （UHPLC-MS） overcomes these limitations and achieves faster separations. This article discusses how an optimized UHPLC-MS method with respect to stationary phase, mobile phase, and detector settings can achieve pictogram-level quantitation of 14 drugs and metabolites employing a 12-min separation. The drugs and metabolites were separated on a perfluorophenyl, 1.9-μm, 100 mm × 2.1 mm column and detected by a fast-scanning single-quadrupole mass spectrometer.