Rapid real-time loop-mediated isothermal amplification combined with coated activated carbon for detection of low numbers of Salmonella enterica from lettuce without enrichment |
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| Affiliation: | 1. Key Laboratory of Mountain Surface Processes and Ecological Regulation, Institute of Mountain Hazards and Environment, Chinese Academy of Sciences, Chengdu 610041, China;2. University of Chinese Academy of Sciences, Beijing 100039, China;1. Natural History Museum of Utah, Department of Anthropology, University of Utah, 301 Wakara Way, Salt Lake City, UT 84108, USA;2. Division of Earth and Ecosystem Sciences, Desert Research Institute, 2215 Raggio Parkway, Reno, NV 89512, USA;3. Texas Archaeological Research Laboratory, University of Texas at Austin, 1 University Station R7500, Austin, TX 78712–0714, USA;4. Metcalf Archaeological Consultants, Inc. and Center for Mountain and Plains Archaeology, Colorado State University, PO Box 2062, Eagle, CO 81631, USA |
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| Abstract: | A real-time loop amplified (Rti-LAMP) DNA assay system was developed for the rapid detection of low numbers of Salmonella enterica ser. Enteritidis (S. enterica) on the leaves of romaine lettuce without enrichment. The assay involved seeding 50 g portions of leaves with various numbers of S. enterica CFU. The lowest level of DNA consistently detected by the Rti-LAMP assay was that from 25 CFU per 50 g (0.5 CFU/g) of lettuce equivalent to the DNA from 6 CFU per Rti-LAMP reaction. A standard curve was generated by plotting the Tp values (min) against the log of 6, 25, 60 and 250 CFU of S. enterica seeded onto 50 g of lettuce. The entire assay could be completed in 3.5 h.7 |
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| Keywords: | Rti-LAMP Lettuce Sodium dodecyl sulfate SDS Bentonite coated activated carbon |
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