Combined effect of carvacrol and citral on the growth of Listeria monocytogenes and Listeria innocua and on the occurrence of damaged cells |
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| Affiliation: | 1. Departamento de Biotecnología Agroalimentaria, Biopolis S.L., Avda. Agustín Escardino, 9, 46980, Paterna, Spain;2. CNPq Postdoctoral Fellowship, Espirito Santo Federal University, Department of Veterinary Medicine, Alegre, Brazil;3. Embrapa Food Technology, Av. das Américas, 23020-470, Rio de Janeiro, RJ, Brazil;4. Department of Preservation and Food Quality, Instituto de Agroquímica y Tecnología de Alimentos (IATA-CSIC), Valencia, Spain;5. Institute of Food Quality and Food Safety, University of Veterinary Medicine, Hannover, Germany;1. Department of Agricultural and Food Sciences, Alma Mater Studiorum, University of Bologna, Campus of Food Science, Piazza Goidanich 60, 47521 Cesena, Italy;2. Interdepartmental Center for Industrial Agri-food Research, University of Bologna, Piazza Goidanich 60, 47521 Cesena, FC, Italy;1. Laboratory of Food Microbiology, Department of Nutrition, Health Sciences Center, Federal University of Paraíba, João Pessoa, Brazil;2. Laboratory of Microbiology, Research Center Aggeu Magalhães (CPqAM), FIOCRUZ, Recife, Brazil;3. Laboratory of Food Biochemistry, Department of Food Engineering, Technology Center, Federal University of Paraíba, João Pessoa, Brazil;4. Laboratory of Genetic of Microorganisms, Department of Molecular Biology, Exact and Nature Sciences Center, Federal University of Paraíba, João Pessoa, Brazil;1. Department of Seafood Processing Technology, Faculty of Fisheries, Cukurova University, 01330, Adana, Turkey;2. Biotechnology Centre, Cukurova University, Adana, Turkey |
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| Abstract: | The aim of this study was to evaluate the growth kinetics of Listeria innocua Serovar 6a (CECT 910) and Listeria monocytogenes Serovar 4b (CECT 4032) exposed to combinations of carvacrol and citral (0.0 μL/mL (control), 0.050 μL/mL of carvacrol and 0.075 μL/mL of citral, 0.050 μL/mL of carvacrol and 0.125 μL/mL of citral, 0.085 μL/mL of carvacrol and 0.075 μL/mL of citral, and 0.085 μL/mL of carvacrol and 0.125 μL/mL of citral), with two initial inoculum concentrations, and also the occurrence of sublethal damage in these cell populations. The terpene combinations exhibited antibacterial activity against L. innocua and L. monocytogenes and the effects were dependent on the concentration of terpenes present in the culture medium (p ≤ 0.05). When terpene-treated L. innocua and L. monocytogenes were incubated in TSB, significant differences in lag phase and growth rate were observed between low and high inoculum concentrations (p ≤ 0.05), indicating that the inoculum level should be taken into account in modeling studies. When bacterial cells were exposed to terpenes the proportion of sublethally injured cells increased with the increase in the terpene dose (p ≤ 0.05). In conclusion, all of these results show that carvacrol and citral can be used in combination at 25% of the MIC in order to control Listeria growth. |
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| Keywords: | Antimicrobials Combined effect Carvacrol Citral Sublethal injury |
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