Stability and mechanical evaluation of bovine pericardium cross-linked with polyurethane prepolymer in aqueous medium |
| |
Authors: | Birzabith Mendoza-Novelo Diego I. Alvarado-Castro José L. Mata-Mata Juan V. Cauich-Rodríguez Arturo Vega-González Eduardo Jorge-Herrero Francisco J. Rojo Gustavo V. Guinea |
| |
Affiliation: | 1. Universidad de Guanajuato, División de Ciencias e Ingenierías, Depto. de Ingenierías Química, Electrónica y Biomédica, Loma del Bosque 103, 37150, León, Gto.,Mexico;2. Universidad de Guanajuato, División de Ciencias Naturales y Exactas, Depto. de Química, Noria alta s/n, 36050, Guanajuato, Gto,Mexico;3. Centro de Investigación Científica de Yucatán A.C., Calle 43 No. 130, Col. Chuburná de Hidalgo, 97200, Mérida, Yucatán, Mexico;4. Hospital Universitario Puerta de Hierro, Servicio de Investigaciones Quirúrgicas, C/Manuel de Falla 2, 28222, Majadahonda, Madrid, Spain;5. Universidad Politécnica de Madrid, E.T.S.I. de Caminos, Canales y Puertos, Depto. de Ciencias de Materiales, C/Profesor Aranguren s/n, 28040 Madrid, Spain |
| |
Abstract: | The present study investigates the potential use of non-catalyzed water-soluble blocked polyurethane prepolymer (PUP) as a bifunctional cross-linker for collagenous scaffolds. The effect of concentration (5, 10, 15 and 20%), time (4, 6, 12 and 24 h), medium volume (50, 100, 200 and 300%) and pH (7.4, 8.2, 9 and 10) over stability, microstructure and tensile mechanical behavior of acellular pericardial matrix was studied. The cross-linking index increased up to 81% while the denaturation temperature increased up to 12 °C after PUP crosslinking. PUP-treated scaffold resisted the collagenase degradation (0.167 ± 0.14 mmol/g of liberated amine groups vs. 598 ± 60 mmol/g for non-cross-linked matrix). The collagen fiber network was coated with PUP while viscoelastic properties were altered after cross-linking. The treatment of the pericardial scaffold with PUP allows (i) different densities of cross-linking depending of the process parameters and (ii) tensile properties similar to glutaraldehyde method. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|