气相色谱-质谱法分析芫荽籽挥发油化学成分

采用水蒸气蒸馏法从新疆产芫荽籽中提取挥发油,收率w=0.78%。用HP-5色谱柱(30m×0.32mm×0.25μm),氢火焰离子化检测器(FID),选取最佳实验条件,用峰面积归一化法测定其相对含量,并用气相色谱-质谱法(GC/MS)对化学成分进行鉴定。共分离出27个峰,鉴定了21个成分,相对含量占挥发油的99.84%。主要成分为脂肪烃、单萜以及它们的含氧衍生物,其中含量较高的有芳樟醇、内-龙脑、α-葑烯、1-甲基-5-异丙烯基-环己烯和(Z)-2,7-二甲基-5-炔-3-辛烯。本方法稳定可靠。

Analysis of Volatile Chemical Components of Coriandrum Sativum L. Seed by Gas Chromatography-Mass Spectrometry

The chemical components of the volatile oil from Xinjiang Coriandrum Sativum L. seed were analyzed by gas chromatography mass spectrometry (GC/MS). The volatile oil was extracted by steam distillation. The extraction yield of volatile oil was w =0.78%. The operating conditions were HP 5 column (30 m×0.32 mm×0.25 μm) and flame ionization detector(FID). The amount of the components from the volatile oil were determinated by peak area normalization method and the components were identified by GC/MS. Twenty one compounds were identified and the amount of the identified components account for 99.84% of all the volatile oil. The main components include aliphatic hydrocarbons, terpenes and their oxygenated derivtives, among which linalool, endo borneol, α fenchene, 1 methyl 5 (1 methylethenyl) cyclohexene and ( Z ) 2,7 dimetheyl 5 yen 3 octen have higher relative contents. The method is reliable and stable.