Biosynthesis of liver glycerolipids from normal and essential fatty acid-deficient rats:3H-glycerol and 1-14C linoleic acid incorporation |
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Authors: | Maria Elena de Tomas Osvaldo Mercuri |
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Affiliation: | (1) Cáteda de Bioquímica, Instituto de Fisiología, Facultad de Ciencias Médicas, Calle 60 y 120, La Plata, Argentina;(2) Present address: Carrera del Investigador Cientifico of the Consejo Nacional de Investigaciones Cientifica y Tecnicas, Argentina |
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Abstract: | Normal and essential fatty acid (EFA)-deficient rats were injected via the portal vein with a labeled solution containing3H-glycerol and 1-14C-linoleic acid during a 1 min period. Livers were immediately frozen, pulverized, and the lipids extracted and fractioned
by thin layer chromatography. The incorporation of3H-glycerol and 1-14C-linoleic acid into the different lipid fractions was measured, and the per cent distribution and specific radioactivity
determined. A parallel increase was found between the specific activity and the amount of3H-glycerol incorporated into 1,2-diglycerides, triglycerides, lecithin and cephalin from EFA-deficient and normal rats. Since
the amount of glycerol in each fraction studied was quite similar in both groups of rats, these findings can explain the increase
in the specific activity observed in the EFA-deficient rats. Nevertheless these facts do not necessarily imply an increased
turnover rate of these molecules, since we do not know the specific radioactivity of the 1,2-diacylglycerol precursors. A
remarkable increase in the specific radioactivity of the14C-linoleic acid incorporated into lipid fractions from EFA-deficient rats compared with control rats was observed. While the
amount of 1-14C-linoleic acid incorporated into neutral lipids was similar in both groups of rats, a statistically significant increase
in the amount of the label incorporated into phospholipids from EFA-deficient rats was observed. These facts suggest an increased
turnover rate of the radiolinoleic acid into phospholipid molecules from EFA-deficient rats via deacylation-reacylation pathway. |
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