Plasma prefractionation methods for proteomic analysis and perspectives in clinical applications |
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| Authors: | Somchai Chutipongtanate Supawat Chatchen Jisnuson Svasti |
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| Affiliation: | 1. Pediatric Translational Research Unit, Department of Pediatrics, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Salaya, Thailand;2. Department of Tropical Pediatrics, Faculty of Tropical Medicine, Mahidol University, Salaya, Thailand;3. Laboratory of Biochemistry, Chulabhorn Research Institute, Krung Thep Maha Nakhon, Thailand;4. Applied Biological Sciences Program, Chulabhorn Graduate Institute, Thailand |
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| Abstract: | Plasma is a rich source of biomarkers with clinical relevance. However, the wide dynamic range of protein concentration hinders the detection of low abundance proteins. Plasma prefractionation methods serve as indispensable tools to reduce plasma complexity, allowing the opportunity to explore tissue‐derived proteins which leak into the circulation. This review summarizes common approaches in plasma prefractionation methods for proteomic analysis and then discusses some considerations in plasma prefractionation for clinical applications, reviewing some examples of its use in clinical situations. |
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| Keywords: | Biomarker Depletion Enrichment Fractionation Plasma |
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