Mass Spectrometric Analysis of Cerebrospinal Fluid Ubiquitin in Alzheimer's Disease and Parkinsonian Disorders |
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| Authors: | Simon Sjödin Oskar Hansson Annika Öhrfelt Gunnar Brinkmalm Henrik Zetterberg Ann Brinkmalm Kaj Blennow |
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| Affiliation: | 1. Department of Psychiatry and Neurochemistry, Institute of Neuroscience and Physiology, The Sahlgrenska Academy at University of Gothenburg, M?lndal, Sweden;2. Clinical Memory Research Unit, Department of Clinical Sciences Malm?, Lund University, Lund, Sweden;3. Memory Clinic, Sk?ne University Hospital, Malm?, Sweden;4. Clinical Neurochemistry Laboratory, Sahlgrenska University Hospital, M?lndal, Sweden;5. Department of Molecular Neuroscience, University College London Institute of Neurology, London, UK;6. UK Dementia Research Institute at UCL, London, UK |
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| Abstract: | 1 Purpose Dysfunctional proteostasis, with decreased protein degradation and an accumulation of ubiquitin into aggregated protein inclusions, is a feature of neurodegenerative diseases. Identifying new potential biomarkers in cerebrospinal fluid (CSF) reflecting this process could contribute important information on pathophysiology. 2 Experimental design A developed method combining SPE and PRM‐MS is employed to monitor the concentration of ubiquitin in CSF from subjects with Alzheimer's disease (AD), Parkinson's disease (PD), and progressive supranuclear palsy (PSP). Four independent cross‐sectional studies are conducted, studies 1–4, including controls (n = 86) and participants with AD (n = 60), PD (n = 15), and PSP (n = 11). 3 Results The method shows a repeatability and intermediate precision not exceeding 6.1 and 7.9%, respectively. The determined LOD is 0.1 nm and the LOQ range between 0.625 and 80 nm . The CSF ubiquitin concentration is 1.2–1.5‐fold higher in AD patients compared with controls in the three independent AD‐control studies (Study 1, p < 0.001; Study 2, p < 0.001; and Study 3, p = 0.003). In the fourth study, there is no difference in PD or PSP, compared to controls. 4 Conclusion and clinical relevance CSF ubiquitin may reflect dysfunctional proteostasis in AD. The described method can be used for further exploration of ubiquitin as a potential biomarker in neurodegenerative diseases. |
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| Keywords: | alzheimer's disease biomarker parkinson's disease progressive supranuclear palsy ubiquitin |
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