Cell density-dependent DNA fragmentation and its suppression by heparin in primary culture of adult rat hepatocytes |
| |
Authors: | S Maeda H Kimura N Koga KH Lin T Saito |
| |
Affiliation: | Department of Chemistry, Government Industrial Research Institute, Nagoya, Japan. |
| |
Abstract: | We show here that the internucleosomal DNA fragmentation, which is a biochemical hallmark of apoptosis, was induced in a cell density-dependent manner in primary culture of adult rat hepatocytes. This DNA fragmentation could be suppressed by a gene expression inhibitor, indicating the active nature of this process. Moreover, the viability changes in high and low cell density cultures showed a tendency corresponding with the incidence of the DNA fragmentation in them. These results suggest that in hepatocytes there may be a cell density-dependent apoptosis mechanism. In this report, we also show that heparin could suppress this DNA fragmentation with high specificity, and the cell death to some extent. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|