A simplified colorimetric micromethod for determination of serum cholesterol |
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| Authors: | Rao R Bhandaru Sathanur R Srinivasan Padmakar S Pargaonkar Gerald S Berenson |
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| Affiliation: | (1) Department of Medicine, Louisiana State University Medical Center, New Orleans, LA |
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| Abstract: | A simple and rapid micromethod is described for quantitation of cholesterol in 5–10 μl of serum using uranyl acetate to deproteinize
the serum ando-phthalaldehyde reagent to develop color. Independent analyses of serum samples by the present method and by two reference
methods, Abell-Kendall and automated colorimetric method, indicated accuracy of the micromethod (r=0.99). Use of capillary
blood specimens makes this procedure applicable for screening infants and small children. |
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