羰基还原酶与甲酸脱氢酶偶联催化制备(R)-苯基乙二醇

以重组菌E. coli Rosetta/pETDuet-rcr-fdh全细胞为催化剂，2-羟基苯乙酮和甲酸钠为双底物，研究了生物催化反应体系中温度、pH值、底物2-羟基苯乙酮的初始浓度、反应时间、细胞浓度和状态等对产物(R)-苯基乙二醇生成效率的影响规律. 结果表明，在温度35℃和pH 7.0、底物初始浓度6 g/L、时间36 h、湿细胞浓度10%(w)的条件下，产物的光学纯度高达98.37% e.e.，得率达79.14%. 在上述反应体系中添加5 mmol/L ZnSO4后，重组菌催化的不对称还原反应效率显著提高，产物(R)-苯基乙二醇光学纯度达到100% e.e.，得率高达86.3%.

Preparation of(R)-1-Phenyl-1,2-ethanediol by Biotransformation with a Coupled System of(R)-Specific Carbonyl Reductase and Formate Dehydrogenase

The effects of reaction temperature,pH value,initial substrate concentration,reaction duration,cell concentration and forms on biotransformation reaction for preparation of(R)-1-phenyl-1,2-ethanediol were studied with 2-hydroxyacetophenone and sodium formate as substrates and the whole cells of E.coli Rosetta/pETDuet-rcr-fdh as catalyst.The results showed that when the concentration of 2-hydroxyacetophenone was 6 g/L,(R)-1-phenyl-1,2-ethanediol was produced with high optical purity of 98.37% enantiomeric ex...