| 丹皮总黄酮的提取及抗氧化活性研究 |
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| 引用本文： | 丁彩真,;于曙光,;王艳丽,;金玉波,;郑国生. 丹皮总黄酮的提取及抗氧化活性研究[J]. 化学与生物工程, 2014, 0(7): 30-33 |
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| 作者姓名： | 丁彩真, 于曙光, 王艳丽, 金玉波, 郑国生 |
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| 作者单位： | [1]青岛农业大学化学与药学院,山东青岛266109; [2]即墨市第二中学,山东即墨266214; [3]青岛农业大学生命科学学院,山东青岛266109 |
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| 基金项目： | 山东省科技发展计划资助项目（2012G0030038） |
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| 摘 要： | 以丹皮为原料提取丹皮总黄酮,并测得其在丹皮中的含量为1.6304%。以常用的化学合成抗氧化剂2,6-二叔丁基-4-甲基苯酚(BHT)和叔丁基对苯二酚(TBHQ)为对照,通过4种方法评价了丹皮总黄酮的抗氧化活性。结果表明,在相同浓度条件下,丹皮总黄酮对羟基自由基(·OH)清除率对DPPH自由基(·DPPH)清除率对超氧阴离子自由基(·O-2)清除率对小鼠肝组织脂质过氧化的抑制率;丹皮总黄酮具有较强的抗氧化能力,其抗氧化活性略低于或等于TBHQ的抗氧化活性,远远大于BHT的抗氧化活性,在较低浓度下即具有对多种氧化反应的抑制作用。表明,丹皮总黄酮是一种很有发展前景的天然抗氧化剂。
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| 关 键 词： | 丹皮总黄酮 提取 抗氧化活性 自由基清除率 |
Study on Extraction and Antioxidant Activities of Total Flavonoids from Tree Peony Bark |
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| Affiliation: | DING Cai-zhen, YU Shu-guang, WANG Yan-li, JIN Yu-bo, ZHENG Guo-sheng (1. College of Chemistry and Pharmacy, Qingdao Agricultural University, Qingdao 266109, China ; 2. Jimo Second Middle School, Jimo 266214, China ;3. College of Life Science, Qingdao Agricultural University, Qingdao 266109, China) |
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| Abstract: | Total flavonoids were extracted from Tree peony bark,whose content accounting for Tree peony bark（dry mass）was 1.6304%.The antioxidant activities of total flavonoids were also studied through four methods with two commonly used antioxidants BHT and TBHQ as control compounds.Results showed that, the total flavonoids had the antioxidant activity following the order as follows：·OH scavenging rate〉·DPPH scavenging rate 〉·O-2 scavenging rate〉the inhibition rate of lipid peroxidation in mouse liver issue.The total flavonoids from Tree peony bark had strong antioxidant activity,which was slightly lower than TBHQ,but far greater than that of BHT.At lower concentration,the total flavonoids can inhibit several oxidation reactions, which showed that total flavonoids from Tree peony bark was a promising natural antioxidant. |
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| Keywords: | total flavonoids from Tree peony bark extraction antioxidant activity the scavenging rate to free radicals |
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