| 普拉格雷含量及有关物质的测定 |
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| 引用本文： | 刘娥. 普拉格雷含量及有关物质的测定[J]. 化学与生物工程, 2014, 0(7): 76-78 |
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| 作者姓名： | 刘娥 |
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| 作者单位： | 荆楚理工学院化工与药学院,湖北荆门448000 |
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| 基金项目： | 湖北省教育厅科研项目（Q20134302） |
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| 摘 要： | 采用HPLC法测定了普拉格雷原料药含量和有关物质。色谱条件如下:色谱柱为C18柱(150mm×4.6mm,5μm),柱温30℃,流动相为乙腈-磷酸盐缓冲溶液(pH值5.0)(50∶50,体积比),流速1.0mL·min-1,检测波长219nm,进样量10μL。结果表明:普拉格雷在0.5~3.0μg·mL-1范围内,线性关系良好,平均加样回收率为99.95%(n=9)。此法操作简便、快速、灵敏、准确,样品处理简便易行,可用于测定普拉格雷原料药含量和有关物质。
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| 关 键 词： | HPLC 普拉格雷 有关物质 含量 |
Determination of the Content of Prasugrel and Its Related Substances |
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| LIU E. Determination of the Content of Prasugrel and Its Related Substances[J]. Chemistry & Bioengineering, 2014, 0(7): 76-78 |
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| Authors: | LIU E |
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| Affiliation: | LIU E (College of Chemical Engineering and Pharmacy ,J ingchu University of Technology, J ingmen 448000, China) |
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| Abstract: | A HPLC method for determination of the content of Prasugrel and its related substances was es-tablished.The chromatographic conditions were conducted as follows：Diamonsil C18 column（150 mm×4.6 mm, 5μm）with the temperature of 30 ℃,mobile phase consisting of acetonitrile∶phosphate buffer （pH value 5.0） in the volume ratio of 50∶50,the flow rate of 1.0 mL·min-1 ,the detection wavelength of 219 nm,the injection volume of 10μL .The calibration curve for Prasugrel was linear in the range of 0.5~3.0μg·mL-1 .The aver-age of recovery rate was 99.95%（n=9）.The method was rapid,simple,accurate,sensitive and suitable for de-termination of the content of Prasugrel and its related substances. |
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| Keywords: | HPLC Prasugrel related substances content |
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