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酶法改性对花生浓缩蛋白吸油性的影响
引用本文:李鹏,杨伟强.酶法改性对花生浓缩蛋白吸油性的影响[J].粮油食品科技,2011,19(5).
作者姓名:李鹏  杨伟强
作者单位:1. 青岛农业大学食品学院,山东青岛,266109
2. 山东省花生研究所,山东青岛,266100
摘    要:花生浓缩蛋白的制备是以低变性花生蛋白粉为原料,通过二次乙醇浸提的方法制得花生浓缩蛋白。用胰蛋白酶对所得花生浓缩蛋白进行改性,通过正交试验得到最佳的改性条件为:料液比1∶11,酶与底物的比例(E∶S)为0.2%,酶解温度35℃,酶解时间30 m in,在此条件下,花生浓缩蛋白的吸油性较未改性前提高了79.6%。

关 键 词:花生蛋白  花生浓缩蛋白  胰蛋白酶  改性  吸油性

Effect of enzymatic modification on the oil absorptionof peanut protein concentrate
LI Peng,YANG Wei-qiang.Effect of enzymatic modification on the oil absorptionof peanut protein concentrate[J].Science and Technology of Cereals,Oils and Foods,2011,19(5).
Authors:LI Peng  YANG Wei-qiang
Affiliation:LI Peng1,YANG Wei-qiang2(1.College of Food Science & Engineering,Qingdao Agricultural University,Qingdao Shandong 266109,2.Shandong Peanut Research Institute,Qingdao Shandong 266100)
Abstract:Peanut protein concentrate was extracted from low-denatured peanut protein flour by ethanol lixiviation twice.The peanut protein concentrate was modified by trypsin.Through orthogonal test,the optimum conditions were obtained as follows:The solid-to-liquid ratio was 1∶11,the E∶S was 0.2%,the reaction time was 30 min,and the reaction temperature was 35 ℃,the oil absorption of peanut protein concentrate after enzymatic modification could be increased by 79.6%.
Keywords:peanut protein  peanut protein concentrate  trypsin  modification  oil absorption
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