冠突散囊菌的分离及其液态发酵特性

从茯砖茶中分离冠突散囊菌并研究其液态发酵特性。通过分析不同培养基组分对冠突散囊菌生长情况的影响,得出液态培养基最佳组成:蔗糖6%、黑茶浸提液0.8%。通过正交试验优化发酵条件,最优发酵条件为:初始-pH值为5.5,培养温度为30℃,孢子接种量为1.5%(孢子浓度5.0～6.0×10~8个/mL),摇瓶转数为120 r/min。在最佳培养基和最优发酵条件下摇瓶培养96 h,菌丝体干重可达0.3998 g/L。

Studies on the Selecting of Eurotium cristatum and It's Liquid Fermentation Characters

In this paper,Eurotium cristaturn strain was selected and it's liquid fermentation characters was investigated.By analysizing the effect of different ingredients of culturemedium on strain's growth,we have identified that optimum culture medium:the concentration of succharose was 6%,and the concentration of dark green tea was 0.8%.The orthogonal experiment was made to optimize fermentation conditions which as follows:initial pH value 5.5,culture temperature 30℃,inoculation volume 1.5%,shaking flask 120r/ min.When the train was cultrued for 96h in optimum culture medium and at the optimized conditions,myce- lium dry weight can reach 0.3998 g/100 mL.