| 抗菌肽Lactoferricin表达载体pG-Lfn的构建 |
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| 引用本文： | 李铁晶,李景鹏,周东坡. 抗菌肽Lactoferricin表达载体pG-Lfn的构建[J]. 食品科学, 2007, 28(7): 286-290 |
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| 作者姓名： | 李铁晶 李景鹏 周东坡 |
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| 作者单位： | 东北农业大学食品学院； 黑龙江大学生命科学学院 黑龙江哈尔滨150030； 黑龙江哈尔滨150030； |
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| 摘 要： | Lactoferricin是一种天然抗菌肽。本实验进行了Lactoferricin基因的PCR合成,将其引入质粒pGEX-6p-1,转化E.coli JM-109,并进行PCR鉴定、酶切鉴定及测序,成功构建了Lactoferricin基因表达载体pG-Lfn。
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| 关 键 词： | Lactoferricin 抗菌肽 表达载体 |
| 文章编号： | 1002-6630（2007）07-0286-05 |
| 修稿时间： | 2006-05-30 |
Construction of Expressing Vector pG-Lfn of Antimicrobial Peptide Lactoferricin |
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| LI Tie-jing,LI Jing-peng,ZHOU Dong-po. Construction of Expressing Vector pG-Lfn of Antimicrobial Peptide Lactoferricin[J]. Food Science, 2007, 28(7): 286-290 |
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| Authors: | LI Tie-jing LI Jing-peng ZHOU Dong-po |
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| Affiliation: | 1.College of Food Science, Northeast Agricultural University, Harbin 150030, China; 2.College of Life Science, Heilongjiang University, Harbin 150030, China |
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| Abstract: | Lactoferricin is a kind of natural antibacterial peptide. In this study, the gene of Lactoferricin was synthesized with PCR, and was linked with the plasmid of pGEX-6p-1, then transformed into E.coli JM-109. The identifications of PCR, enzymical degestion and sequencing were carried out, and the expressing vector of pG-Lfn was successfully constructed. |
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| Keywords: | Lactoferricin |
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