Detection of Foot‐and‐Mouth Disease Virus Using a Polydiacetylene Immunosensor on Solid‐Liquid Phase |
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| Authors: | Jae‐Pil Jeong Eunae Cho Soo‐Chan Lee Taejoon Kim Bokyeong Song Im‐Soon Lee Seunho Jung |
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| Affiliation: | 1. Department of Systems Biotechnology, Microbial Carbohydrate Resource Bank (MCRB), Center for Biotechnology Research in UBITA (CBRU), Konkuk University, Gwangjin‐gu, Seoul, South Korea;2. Center for Biotechnology Research in UBITA (CBRU), Institute for Ubiquitous Information Technology and Applications (UBITA), Konkuk University, Gwangjin‐gu, Seoul, South Korea;3. Department of Biological Sciences, Konkuk University, Gwangjin‐gu, Seoul, South Korea |
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| Abstract: | The rapid detection of foot‐and‐mouth disease virus (FMDV) is vital for the prevention of foot‐and‐mouth disease outbreaks. In this study, a polyvinylidene difluoride (PVDF)‐supported polydiacetylene (PDA) immunosensor is developed to detect FMDV, in which a polyclonal antibody against the FMDV VP1 antigen is conjugated as a specific virus‐binding module without a linker. First, a liposome‐based immunosensor is generated for the FMDV VP1 protein in the form of photopolymerized PDA colloids. Then, the VP1‐specific PDA immunosensors are modified onto PVDF strip to enable the rapid and portable detection of FMDV. Detailed analyses are performed using ultraviolet‐visible spectroscopy, dynamic light scattering, transmission electron microscopy, and scanning electron microscopy. A blue‐to‐red color transition is observed in the presence of FMDV particles, indicating the potential applications of FMDV‐specific PDA immunosensors for use in solid‐phase detection as well as via liquid‐phase liposome platforms. Thus, this work provides a rapid and simple detection for FMDV. |
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| Keywords: | antibody– antigen interaction foot‐and‐mouth disease virus (FMDV) polydiacetylene (PDA) polyvinylidene difluoride (PVDF) |
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