Ascorbic acid imprinted polymer-modified graphite electrode: A diagnostic sensor for hypovitaminosis C at ultra trace ascorbic acid level |
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Authors: | Bhim Bali Prasad Deepak Kumar Rashmi Madhuri Mahavir Prasad Tiwari[Author vitae] |
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Affiliation: | Analytical Division, Department of Chemistry, Faculty of Science, Banaras Hindu University, Varanasi 221005, India |
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Abstract: | A new kind of molecularly imprinted polymer-modified graphite electrode was fabricated by “grafting-to” approach, incorporating sol–gel technique, for the detection of acute deficiency in serum ascorbic acid level (SAAL), manifesting hypovitaminosis C. The modified electrode exhibited ascorbic acid (AA) oxidation at less positive potential (0.0 V) than the earlier reported methods, resulting in a limit of detection as low as 6.13 ng mL−1 (RSD = 1.2%, S/N = 3). The diffusion coefficient (1.096 × 10−5 cm2 s−1), rate constant (7.308 s−1), and Gibb's free energy change (−12.59 kJ mol−1) due to analyte adsorption, were also calculated to explore the kinetics of AA oxidation. The proposed sensor was found to enhance sensitivity substantially so as to detect ultra trace level of AA in the presence of other biologically important compounds (dopamine, uric acid, etc.), without any cross interference and matrix complications from biological fluids and pharmaceutical samples. |
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Keywords: | Ascorbic acid Differential pulse anodic striping voltammetry Molecularly imprinted polymer Sol&ndash gel &ldquo Grafting-to&rdquo approach |
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