A photoluminescence-based quantum semiconductor biosensor for rapid in situ detection of Escherichia coli |
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Authors: | Valé rie Duplan,Eric Frost,Jan J. Dubowski[Author vitae] |
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Affiliation: | aLaboratory for Quantum Semiconductors and Photon-based BioNanotechnology, Department of Electrical and Computer Engineering, Faculty of Engineering, Université de Sherbrooke, Sherbrooke, Québec J1K 2R1, Canada;bDepartment of Microbiology and Infectiology, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, Québec J1H 5N4, Canada |
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Abstract: | This work describes a novel method of detecting Escherichia coli using photoluminescence (PL) emission from III–V quantum semiconductor (QS) devices functionalized with two different antibody-based architectures. The first approach employed self-assembled monolayers of biotinylated polyethylene glycol thiols to immobilize biotinylated antibody via neutravidin. In the second approach, we used QS microstructures coated with a thin layer of Si3N4 allowing direct functionalization with E. coli antibodies through hydrofluoric acid etching and glutaraldehyde-based reticulation. Atomic force, optical and fluorescence microscopy measurements were used to assess the immobilization process. Depending on the biosensing architecture, density of the immobilized bacteria was observed in the range of 0.5–0.7 bacteria/100 μm2. The detection of E. coli at 104 CFU/ml was achieved within less than 120 min of the bacteria exposure. It is expected that an even better sensitivity threshold could be achieved following further optimization of the method. |
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Keywords: | Quantum semiconductors GaAs Photoluminescence Optical biosensor Self-assembled monolayers Escherichia coli |
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