| SPF鸡肠源嗜酸乳杆菌的分离及生物学特性研究 |
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| 引用本文： | 颜世敢,朱丽萍,曹洪敬,任素芳,徐培莲,刘俊珍.SPF鸡肠源嗜酸乳杆菌的分离及生物学特性研究[J].山东轻工业学院学报,2003,17(4):46-50. |
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| 作者姓名： | 颜世敢 朱丽萍 曹洪敬 任素芳 徐培莲 刘俊珍 |
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| 作者单位： | [1]山东省农业科学院畜牧兽医研究所。山东济南250100 [2]山东轻工业学院食品与生物工程学院。山东济南250100 [3]农业部动物检疫所，山东青岛266063 |
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| 基金项目： | 山东省科技攻关项目(981041103) |
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| 摘 要： | 无菌采集SPF鸡盲肠粘膜层内容物．接种MRS培养基和SL乳杆菌选择培养基，厌氧培养分离乳杆菌。对分离菌从形态学、生化特性及耐氧性、产酸性、耐酸性、药物敏感性、抑菌性、粘附性、致病性等生物学特性研究。实验结果对照《伯杰氏细菌学鉴定手册》(第9版)的参数综合评判。结果表明从SPF鸡盲肠内分离到2株嗜酸乳杆菌，从中筛选出一株适宜研制家禽微生态制剂的菌种。
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| 关 键 词： | SPF鸡 肠源嗜酸乳杆菌 分离 生物学特性 厌氧培养 家禽用微生态制剂 |
| 文章编号： | 1004-4280(2003)04-0046-05 |
| 修稿时间： | 2003年6月6日 |
Study on isolation and biology characteristic of Lactobacillus acidophilus from SPF chicken's cecum |
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| YAN Shi-gan,ZHU Li-ping,CAO Hong-jing,REN Su-fang,XU Pei-lian,LIU Jun-zhen.Study on isolation and biology characteristic of Lactobacillus acidophilus from SPF chicken''''s cecum[J].Journal of Shandong Institute of Light Industry(Natural Science Edition),2003,17(4):46-50. |
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| Authors: | YAN Shi-gan ZHU Li-ping CAO Hong-jing REN Su-fang XU Pei-lian LIU Jun-zhen |
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| Affiliation: | YAN Shi-gan~1,ZHU Li-ping~2,CAO Hong-jing~3,REN Su-fang~1,XU Pei-lian~3,LIU Jun-zhen~1 |
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| Abstract: | Introduction was made to asepsisly scraping content from mucosa of SPF chicken's cecum,inoculating MRS culture medium and SL culture medium,separating lactobacillus by use of inoxygen culturing.Study was conducted on form character,biochemical character,oxygen enduring,acidity producing ability,acidity enduring,medicine sensitivity,bacterioatasis,adhesion,pathogenic ability and so on of the isolated bacteria.Results were compared with parameters referred to Bergey's Manual of Systematic Bacteriology(9~(th) edition).Finally identified were 2 strains of Lactobacillus acidophilus one of which was suitable to prepare probiotics to be used in chicken. |
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| Keywords: | SPF chicken Lactobacillus acidophilus isolate biology characteristic |
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