柱层析分离红曲色素及其组分的抑菌性对比

实验采用了硅胶柱层析方法,对红曲色素中包含的红曲红、红曲橙、红曲黄三种组分进行分离条件研究,结果表明：应使用梯度洗脱的方法对红曲色素三种组分进行分离,正己烷：乙酸乙酯混合液（体积比5：1）为黄色组分洗脱剂;乙酸乙酯为橙色组分洗脱剂;乙酸乙酯：甲醇混合液（体积比5：1）为红色组分洗脱剂;当分离条件为：红曲色素上样量2mL,洗脱剂流速3mL/min时,分离效果最好。并分别进行三种分离组分对大肠杆菌和枯草芽胞杆菌的抑菌性试验,得到橙色组分对其两种菌的抑菌性最强。

Separation of Monascus Pigments by Column Chromatography and Inhibition Comparison of Isolated Components

In this study,silica gel column chromatography was used to separate red pigments,orange pigments and yellowpigments,the three components of monascus pigments.And the separating conditions were researched and determined.The results showed that separating the three components of pigment components should make use of gradient elution method.The mixture of n-hexane and ethylacetate（volume ratio of 5：1） for isolating yellow components,ethylacetate for isolating orange components and the mixture of ethylacetate and methanol（volume ratio of 5：1） for isolating red components.The best sample was 2 mL,the best elution agents velocity was 3 mL/min.And then comparing the antibacterial activity of three components separated from pigment to E.coli and Bacillus subtilis.The results showed that the antibacterial activity orange components is strongest in the three components.